201919th International Congress of Metrology
|Number of page(s)||9|
|Section||Nanometrology / Nanometrologie|
|Published online||23 September 2019|
Asymmetric field flow fractionation applied to the nanoparticles characterization: Study of the parameters governing the retention in the channel
Department of Biomedical and Inorganic Chemistry, Laboratoire National de Métrologie et d’Essai (LNE), 1 rue Gaston Boissier, Paris, 75015, France
2 Laboratoire de Physique et Mécanique des Milieux Hétérogènes (PMMH), ESPCI Paris, CNRS, 10 rue Vauquelin, 75231, Paris Cedex 05, France
In this work we study the parameters which are often modified to optimize the separation in asymmetrical field flow fractionation, and we observe their impact on the retention behavior of the analyte. The aim of the work is to improve our knowledge of the phenomena which govern the behavior of the analytes in the channel and to have a better understanding of the limits of the actual theoretical model in order to improve it. Results illustrate that the ionic strength influences the effect of the cross flow rate on the retention time of the particle. The question of the determination of the channel thickness was also addressed.
© The Authors, published by EDP Sciences, 2019
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.