Metrology in Thermal cyclers: a new approach
1 APoDis Technologies S.A.S, RD 2020 Locon, 41600 Chaumon-sur-Tharonne, France
2 Tumorothèque Caen Basse Normandie, 3 avenue du Général Harris 14076 Caen cedex 5
Metrological laboratories propose different protocols to qualify thermal cyclers, each depending on a different approach. But all these protocols may not match the laboratory need. Some questions must be answered before any metrological test. Has the qualification protocol be performed with a fixed temperature program? What must be done if the metrological qualification is out of specification? Can specifications be set already?
With reference to standardised publications [1-3], we are going to try to focus on the current state of art about metrological qualification. It seems to be necessary to confirm any metrological test by a biological one. And to conclude that a difference between measured value and fixed value does not necessary lead to an end-of-life your thermal cycler even a false genetic analysis.
© The Authors, published by EDP Sciences, 2017
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