| Numéro |
2013
16th International Congress of Metrology
|
|
|---|---|---|
| Numéro d'article | 14007 | |
| Nombre de pages | 4 | |
| Section | Métrologie sensorielle / Soft metrology | |
| DOI | https://doi.org/10.1051/metrology/201314007 | |
| Publié en ligne | 7 octobre 2013 | |
Hyperspectral imaging: a tool for biological measurements
INRIM Istituto Nazionale di Ricerca Metrologica, 10135 Strada delle Cacce 91, Turin, Italy
a Corresponding author: v.caricato@inrim.it
We present a hyperspectral imaging device (HSI) based on a scanning Fabry-Perot interferometer able to build a hyperspectral cube with high photon collection efficiency (throughput). The device allows to obtain a single image of a sample treated with different fluorescence substances and to unambiguously discriminate them by observing their spectral fingerprint. Furthermore it allows studying biological samples with colorimetric analysis. An experiment carried out with cyanines, fluorescein and quantum dots emitting in the yellow-orange region demonstrates the potentiality of multi-labeled fluorescence microscopy without the use of multiple filter set or dispersive means. The spectra of some biological samples have been computed to demonstrate the validity of the HSI device coupled with an inverted microscope.
© Owned by the authors, published by EDP Sciences, 2013
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 2.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.